Optimal Exploitation of the Sentinel-2 Spectral Capabilities for Crop Leaf Area Index Mapping

The continuously increasing demand of accurate quantitative high quality information on land surface properties will be faced by a new generation of environmental Earth observation (EO) missions. One current example, associated with a high potential to contribute to those demands, is the multi-spectral ESA Sentinel-2 (S2) system. The present study focuses on the evaluation of spectral information content needed for crop leaf area index (LAI) mapping in view of the future sensors. Data from a field campaign were used to determine the optimal spectral sampling from available S2 bands applying inversion of a radiative transfer model (PROSAIL) with look-up table (LUT) and artificial neural network (ANN) approaches. Overall LAI estimation performance of the proposed LUT approach (LUTN₅₀) was comparable in terms of retrieval performances with a tested and approved ANN method. Employing seven- and eight-band combinations, the LUTN₅₀ approach obtained LAI RMSE of 0.53 and normalized LAI RMSE of 0.12, which was comparable to the results of the ANN. However, the LUTN50 method showed a higher robustness and insensitivity to different band settings. Most frequently selected wavebands were located in near infrared and red edge spectral regions. In conclusion, our results emphasize the potential benefits of the Sentinel-2 mission for agricultural applications

Using a Remote Sensing-Supported Hydro-Agroecological Model for Field-Scale Simulation of Heterogeneous Crop Growth and Yield: Application for Wheat in Central Europe

The challenge of converting global agricultural food, fiber and energy crop cultivation into an ecologically and economically sustainable production process requires the most efficient agricultural management strategies. Development, control and maintenance of these strategies are highly dependent on temporally and spatially continuous information on crop status at the field scale. This paper introduces the application of a process-based, coupled hydro-agroecological model (PROMET) for the simulation of temporally and spatially dynamic crop growth on agriculturally managed fields. By assimilating optical remote sensing data into the model, the simulation of spatial crop dynamics is improved to a point where site-specific farming measures can be supported. Radiative transfer modeling (SLC) is used to provide maps of leaf area index from Earth Observation (EO). These maps are used in an assimilation scheme that selects closest matches between EO and PROMET ensemble runs. Validation is provided for winter wheat (years 2004, 2010 and 2011). Field samples validate the temporal dynamics of the simulations (avg. R-2 = 0.93) and > 700 ha of calibrated combine harvester data are used for accuracy assessment of the spatial yield simulations (avg. RMSE = 1.15 t center dot ha(-1)). The study shows that precise simulation of field-scale crop growth and yield is possible, if spatial remotely sensed information is combined with temporal dynamics provided by land surface process models. The presented methodology represents a technical solution to make the best possible use of the growing stream of EO data in the context of sustainable land surface management

Using a Remote Sensing-Supported Hydro-Agroecological Model for Field-Scale Simulation of Heterogeneous Crop Growth and Yield: Application for Wheat in Central Europe

The challenge of converting global agricultural food, fiber and energy crop cultivation into an ecologically and economically sustainable production process requires the most efficient agricultural management strategies. Development, control and maintenance of these strategies are highly dependent on temporally and spatially continuous information on crop status at the field scale. This paper introduces the application of a process-based, coupled hydro-agroecological model (PROMET) for the simulation of temporally and spatially dynamic crop growth on agriculturally managed fields. By assimilating optical remote sensing data into the model, the simulation of spatial crop dynamics is improved to a point where site-specific farming measures can be supported. Radiative transfer modeling (SLC) is used to provide maps of leaf area index from Earth Observation (EO). These maps are used in an assimilation scheme that selects closest matches between EO and PROMET ensemble runs. Validation is provided for winter wheat (years 2004, 2010 and 2011). Field samples validate the temporal dynamics of the simulations (avg. R-2 = 0.93) and > 700 ha of calibrated combine harvester data are used for accuracy assessment of the spatial yield simulations (avg. RMSE = 1.15 t center dot ha(-1)). The study shows that precise simulation of field-scale crop growth and yield is possible, if spatial remotely sensed information is combined with temporal dynamics provided by land surface process models. The presented methodology represents a technical solution to make the best possible use of the growing stream of EO data in the context of sustainable land surface management

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Spaceborne Imaging Spectroscopy for Sustainable Agriculture: Contributions and Challenges

Agriculture faces the challenge of providing food, fibre and energy from limited land resources to satisfy the changing needs of a growing world population. Global megatrends, e.g., climate change, influence environmental production factors; production and consumption thus must be continuously adjusted to maintain the producer–consumer-equilibrium in the global food system. While, in some parts of the world, smallholder farming still is the dominant form of agricultural production, the use of digital information for the highly efficient cultivation of large areas has become part of agricultural practice in developed countries. Thereby, the use of satellite data to support site-specific management is a major trend. Although the most prominent use of satellite technology in farming still is navigation, Earth Observation is increasingly applied. Some operational services have been established, which provide farmers with decision-supporting spatial information. These services have mostly been boosted by the increased availability of multispectral imagery from NASA and ESA, such as the Landsat or Copernicus programs, respectively. Using multispectral data has arrived in the agricultural commodity chain. Compared to multispectral data, spectrally continuous narrow-band sampling, often referred to as hyperspectral sensing, can potentially provide additional information and/or increased sampling accuracy. However, due to the lack of hyperspectral satellite systems with high spatial resolution, these advantages mostly are not yet used in practical farming. This paper summarizes where hyperspectral data provide additional value and information in an agricultural context. It lists the variables of interest and highlights the contribution of hyperspectral sensing for information-driven agriculture, preparing the application of future operational spaceborne hyperspectral missions.</p